产品货号：
GS2133
中文名称：
Bse8I内切酶
英文名称：
Bse8I
产品规格：
500U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration(more than 5-fold overdigestion on 1μgsubstrate)may result in star activity.
技术规格
相关搜索：Bse8I内切酶，Bse8I限制性内切酶
使用说明
High enzyme concentration(more than 5-fold overdigestion on 1μgsubstrate)may result in star activity.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl. |
| Recognition sites | GATNN↑NNATC |
| 来源 | Bacillus species 8 |
| Assayed on | Lambda DNA |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
| Optimal temperature | 60℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme about 80%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | Long incubation not recommended owing to ℃currence of star activity |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BsaBI,BseJI,BsiBI,BsrBRI,MamI |
相关搜索：Bse8I内切酶，Bse8I限制性内切酶